Note: Proteins should be diluted in the buffer in which they are dissolved. Bradford assay dye reagent (available commercially, e.g., Bio-Rad Protein Assay Dye Reagent Concentrate, cat.The BSA standard curve should be prepared using the same buffer. The Bradford method is a quantitative protein assay method, based on the binding of a dye, Coomassie Brilliant Blue, to a protein sample, and comparing this binding to a standard curve generated by the reaction of known amounts of a standard protein, usually BSA.įor this assay, protein samples should be diluted in an appropriate buffer (generally the same buffer in which they are dissolved). Looking for more study tips and notes? Follow Leverage Edu on Instagram, Facebook and Twitter or subscribe to our newsletter.Quantifying proteins using the Bradford method Eye Disorders in Children and Adolescents.Study on Probiotics and their Preparation.The ability of Curry and Cinnamon to Inhibit Bacterial Growth.To Study the Coagulable And Non-Coagulable Milk Proteins.To Study of Drug Resistance In Bacteria Using Antibiotics.Quantitative analysis of phytoplankton in a water body.Investigating The Biochemical(also called biological oxygen demand ) of a water sample as a pollution indicator. Impact of local industry on the environment and the remedial measures taken by the industry.Inventory of birds in your locality, their ecological role as scavengers, pollinators, etc.Inventory of weeds in aquatic bodies/agricultural fields.Study Of The Genetic Markers In The Human Population.Study The Effect Of A Local Industry On Environment.To make an inventory of local tree, shrub and herb.The ecological role of some animals observed in a local area.Other Popular Biology Projects for Class 12 Graph your results with a bar graph.Īnalysis and Interpretation: (Prepare a pie chart and write your interpretation based on your result)Ĭonclusion: After DNA replication, onion cells go through different stages of mitosis. Once you have counted at least 1000 cells, convert your tick marks to numbers.Now, count the cells in each stage and record them using tick marks.Once you’re able to identify the different stages of mitosis, draw them.Observe the slide under a microscope, first at the lowest magnification and then at the highest.Put the coverslip on and soak excess dye using a paper towel. Using a dropper, put 2-3 drops of Toluidine blue and wait for 2 minutes.Using a scalpel, trim the roots to 2mm and then using a needle slice it into 2 or more lengthwise sections.Using a dropper, put 3-4 drops of distilled water over it. Use forceps to remove the root tip from the acid and place it on a microscope slide.Allow the roots to soak in the acid for 20 mins Put the root tip in a beaker and pour muriatic acid in it.Once the roots have grown, remove the onion from the water and cut about 5mm off the tips of the roots using a scalpel.Wait for a few days for the roots to grow Place 3-4 toothpicks inside the onion and put it in a jar of water with the onion root side down.Materials Required: Universal indicator, litmus paper and different water samples. Likewise, a pH of more than 8.5 is too basic for the survival of most organisms. Below the pH of 4.5, fishes die and only a small number of acid-tolerant insects survive. If the pH is 3 to 5 it is too acidic for the survival of most organisms. The permissible pH of the water that is safe for our life and health is 6.5-8.5. It is important to monitor the chemical properties of the water we drink or use every day. Other Popular Biology Projects for Class 12īiology Projects for Class 12 Investigating the pH of a water sample.Format of Writing Biology Project for class 12.How Does Microwave Radiation Affect Different Organisms.Does the Color of Light Affect Plant Growth.Factors Affecting Nodule Formation in Legumes.To study the effect of pH on seed germination.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |